immobilised metal ion affinity-based fluorescence polarisation assay

initialism: IMAP™
synonym: IMAP™ assay
https://doi.org/10.1351/goldbook.14149
Assay in which the interaction between nanoparticles coated with triply charged metal ions and phosphate groups on a substrate that carries a fluorescent tag is detected.
Notes:
  1. The phosphorylated fluorescent substrate bound to the nanoparticle will exhibit high fluorescence polarization relative to the unbound (unphosphorylated) peptide substrate.
  2. Used for kinase assays where a phosphate group is added to a peptide substrate or for phosphatase assays where the phosphate is removed from a peptide substrate.
See also: fluorescence polarization
Source:
PAC, 2011, 83, 1129. (Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)) on page 1143 [Terms] [Paper]
Citation: 'immobilised metal ion affinity-based fluorescence polarisation assay' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. https://doi.org/10.1351/goldbook.14149 RIS BibTex EndNote
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