14149 IUPAC Gold Book - immobilised metal ion affinity-based fluorescence polarisation assay 10.1351/goldbook.14149 14149 current initialism: IMAP™ mentioned: IMAP, IMAP assay synonym: IMAP™ assay 1 Assay in which the interaction between nanoparticles coated with triply charged metal ions and phosphate groups on a substrate that carries a fluorescent tag is detected. The phosphorylated fluorescent substrate bound to the nanoparticle will exhibit high fluorescence polarization relative to the unbound (unphosphorylated) peptide substrate. Used for kinase assays where a phosphate group is added to a peptide substrate or for phosphatase assays where the phosphate is removed from a peptide substrate. fluorescence polarization https://goldbook.iupac.org//terms/view/14135 PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1143 (https://doi.org/10.1351/PAC-REC-09-05-03) https://goldbook.iupac.org/terms/view/14149/html https://goldbook.iupac.org/terms/view/14149/json https://goldbook.iupac.org/terms/view/14149/plain Citation: 'immobilised metal ion affinity-based fluorescence polarisation assay' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.14149 The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/) for individual terms. If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org . The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using. 2026-06-28T12:25:37+00:00