fluorescence polarisation

symbol: $p$
https://doi.org/10.1351/goldbook.14135
Dimensionless value determined from the fluorescence intensities (\(I\)) measured parallel (\(I_{\Vert}\)) and perpendicular (\(I_{\perp}\)) to the excitation plane: \[p = \frac{I_{\Vert} - I_{\perp}}{I_{\Vert} + I_{\perp}}\]
Note: Fluorescence anisotropy (\(r\)) differs only in normalization and is determined from \[r = (I_{\Vert} - I_{\perp})/(I_{\Vert} + 2I_{\perp})\]
See also: fluorescence polarization assay
Source:
PAC, 2011, 83, 1129. (Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)) on page 1141 [Terms] [Paper]
Citation: 'fluorescence polarisation' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. https://doi.org/10.1351/goldbook.14135 RIS BibTex EndNote
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