Specifically-designed single-stranded nucleic acid with a defined (known) nucleotide sequence immobilised on a surface.
Notes: - Capture probes are utilised as a recognition element to test the nucleotide sequence of target deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) in the sample solution by using DNA or RNA hybridization.
- An analogous principle, based on immobilised nucleic acid probes, has been applied in studies of the interactions of nucleic acids with proteins or other targets. See also nucleic acid aptamers.
Examples: Nucleic acids: ssDNA (alternatively, RNA or a synthetic nucleic acid analogue, such as locked nucleic acid (LNA) or
peptide nucleic acid (PNA)). Surfaces: microarray, magnetic, or other micro/nanoparticle, a transducer of a biosensor.
Source:
PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1183 (https://doi.org/10.1515/pac-2016-1120)