Approach to identify proteins and characterise their amino acid sequences or post-translational modifications either by direct mass spectrometric analysis of an ionised protein molecule or by sequential combination of protein/peptide fragmentation in the ion source or mass analyser with ion trapping mass spectrometers.
See: bottom-up proteomics
Source:
PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1165 (https://doi.org/10.1515/pac-2016-1120)