https://doi.org/10.1351/goldbook.09759
Mass spectrometric technique that serves to generate peptide fragment ions through the fragmentation of peptide precursor ions via different physicochemical processes in an ion source.
Note: This approach based on the top-down proteomics is an alternative to bottom-up proteomics. The main advantages include the potential for \(\pu{100\%}\) sequence coverage of the protein and improved detection of post-translational modifications.