https://doi.org/10.1351/goldbook.09735
Approach to identify proteins and characterise their amino acid sequences or post-translational modifications by sequential combination of proteolysis (enzymatic proteolysis and chemical proteolysis) prior to mass spectrometric analysis of the resulting proteolytic peptides.
Note: In shotgun proteomics, the proteins in a mixture are digested, usually by a specific enzyme, and the resulting peptides are separated by liquid chromatography and identified by tandem mass spectrometry.
See: top-down proteomics