blocking

https://doi.org/10.1351/goldbook.09666

Coating of an immunospecific surface (e.g. serum, tissues, cells, biological macromolecules, biosensor surface) to form an active cover with selectivity or an inert layer.

Note: Blocking is a pre-treatment method with blocking agents (e.g. monovalent Fab fragment, i.e. the antigen-binding fragment of an immunoglobulin molecule; mono or divalent antibodies; organic ligands; metals).
Source:
PAC, 2018, 90, 1121. (Terminology of bioanalytical methods (IUPAC Recommendations 2018)) on page 1137 [Terms] [Paper]
Citation: 'blocking' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. https://doi.org/10.1351/goldbook.09666 RIS BibTex EndNote
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