Coating of an immunospecific surface (e.g. serum, tissues, cells, biological macromolecules, biosensor surface) to form an active cover with selectivity or an inert layer.
Note: Blocking is a pre-treatment method with blocking agents (e.g. monovalent Fab fragment, i.e. the antigen-binding fragment of an immunoglobulin molecule; mono or divalent antibodies; organic ligands; metals).
Source:
PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1137 (https://doi.org/10.1515/pac-2016-1120)