luminescence assay

https://doi.org/10.1351/goldbook.14160
Assay in which response is measured by the detection of light of a nonradiative origin, such as bioluminescence or chemiluminescence.
Example: A luciferase reporter system for the measurement of gene transcription regulation in which luciferase, transcribed under the regulation of a promoter, oxidizes the substrate luciferin to oxyluciferin. In this process, light of wavelength \(\pu{560 nm}\) is emitted and detected as the signal.
Source:
PAC, 2011, 83, 1129. (Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)) on page 1147 [Terms] [Paper]
Citation: 'luminescence assay' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. https://doi.org/10.1351/goldbook.14160 RIS BibTex EndNote
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