{"term":{"id":"14160","title":"luminescence assay","longtitle":"IUPAC Gold Book - luminescence assay","doi":"10.1351\/goldbook.14160","code":"14160","status":"current","definitions":[{"id":1,"text":"Assay in which response is measured by the detection of light of a nonradiative origin, such as bioluminescence or chemiluminescence.","exams":{"1":"A luciferase reporter system for the measurement of gene transcription regulation in which luciferase, transcribed under the regulation of a promoter, oxidizes the substrate luciferin to oxyluciferin. In this process, light of wavelength \\(\\pu{560 nm}\\) is emitted and detected as the signal."},"sources":["PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1147 (https:\/\/doi.org\/10.1351\/PAC-REC-09-05-03)"]}],"altoutputs":{"html":"https:\/\/goldbook.iupac.org\/terms\/view\/14160\/html","xml":"https:\/\/goldbook.iupac.org\/terms\/view\/14160\/xml","plain":"https:\/\/goldbook.iupac.org\/terms\/view\/14160\/plain"},"citation":"Citation: 'luminescence assay' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351\/goldbook.14160","license":"The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https:\/\/creativecommons.org\/licenses\/by-sa\/4.0\/) for individual terms.","collection":"If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org .","disclaimer":"The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using.","accessed":"2026-06-28T12:28:33+00:00"}}