https://doi.org/10.1351/goldbook.14142
Assay in which response is determined after the physical separation of assay components using high-performance liquid chromatography (HPLC) with concurrent detection, usually by monitoring absorbance at an appropriate wavelength.
Note: Changes in the concentration of assay components at one or more times over the course of the assay can be determined by the relative area under the detection peaks as compared to assay controls and standards.