14142 IUPAC Gold Book - high-performance liquid chromatography assay 10.1351/goldbook.14142 14142 current initialism: HPLC synonym: HPLC Assay 1 Assay in which response is determined after the physical separation of assay components using high-performance liquid chromatography (HPLC) with concurrent detection, usually by monitoring absorbance at an appropriate wavelength. Changes in the concentration of assay components at one or more times over the course of the assay can be determined by the relative area under the detection peaks as compared to assay controls and standards. PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1142 (https://doi.org/10.1351/PAC-REC-09-05-03) https://goldbook.iupac.org/terms/view/14142/html https://goldbook.iupac.org/terms/view/14142/json https://goldbook.iupac.org/terms/view/14142/plain Citation: 'high-performance liquid chromatography assay' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.14142 The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/) for individual terms. If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org . The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using. 2026-06-28T12:25:39+00:00