fluorescence polarisation assay

https://doi.org/10.1351/goldbook.14136
Assay in which the association between an intrinsically fluorescent ligand, or a ligand linked to a fluorescent probe, and a larger macromolecule is detected.
Note: The degree to which the labeled ligand depolarizes plane-polarized light is directly proportional to the rate at which it tumbles in solution. Tumbling will be slower when the ligand is associated with the larger macromolecule as compared to free in solution, consequently resulting in a lower degree of depolarization.
See also: fluorescence polarization, homogeneous binding assay
Source:
PAC, 2011, 83, 1129. (Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)) on page 1141 [Terms] [Paper]
Citation: 'fluorescence polarisation assay' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. https://doi.org/10.1351/goldbook.14136 RIS BibTex EndNote
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