https://doi.org/10.1351/goldbook.14127
Assay in which light emitted by electrogenerated luminescence is detected and used to quantify a label, e.g., ruthenium(II) tris-(bipyridine), on one of the two assay binding components.
Example: Coated magnetic beads bind the protein target and are then captured in a flow cell of the detector where the energy state of the \(\ce{Ru}\) is chemically converted to release a photon measured at \(\pu{620 nm}\) with a photomultiplier tube. There is little or no compound interference during detection because of the wash step within the flow cell. Generally not used for high-throughput screening but for secondary assays.