{"term":{"id":"14127","title":"electrochemiluminescence assay","longtitle":"IUPAC Gold Book - electrochemiluminescence assay","doi":"10.1351\/goldbook.14127","code":"14127","status":"current","definitions":[{"id":1,"text":"Assay in which light emitted by electrogenerated luminescence is detected and used to quantify a label, e.g., ruthenium(II) tris-(bipyridine), on one of the two assay binding components.","exams":{"1":"Coated magnetic beads bind the protein target and are then captured in a flow cell of the detector where the energy state of the \\(\\ce{Ru}\\) is chemically converted to release a photon measured at \\(\\pu{620 nm}\\) with a photomultiplier tube. There is little or no compound interference during detection because of the wash step within the flow cell. Generally not used for high-throughput screening but for secondary assays."},"sources":["PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1137 (https:\/\/doi.org\/10.1351\/PAC-REC-09-05-03)"]}],"altoutputs":{"html":"https:\/\/goldbook.iupac.org\/terms\/view\/14127\/html","xml":"https:\/\/goldbook.iupac.org\/terms\/view\/14127\/xml","plain":"https:\/\/goldbook.iupac.org\/terms\/view\/14127\/plain"},"citation":"Citation: 'electrochemiluminescence assay' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351\/goldbook.14127","license":"The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https:\/\/creativecommons.org\/licenses\/by-sa\/4.0\/) for individual terms.","collection":"If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org .","disclaimer":"The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using.","accessed":"2026-07-24T23:54:38+00:00"}}