https://doi.org/10.1351/goldbook.09939
Separation in which laminar flow conditions are established between a liquid containing suspended sample and a carrier liquid, with a physical force field applied across the streams.
Notes:
- Although SPILTT is similar to field-flow fractionation, it should not be referred to as FFF. The separation is achieved by the combined action of controlled flow rates and an applied cross-field.
- The system has two channel inlets and two outlets. Sample suspension is continuously pumped into one inlet and carrier liquid is pumped into the other inlet. Due to the laminar flow conditions, these two streams do not mix.
- SPLITT enables the separation of samples of masses of the order of grams.
- Fields that have been tested so far include gravitational, centrifugal and magnetic.
- A semi-preparative binary separation can be generated with smaller particles eluting from one outlet and larger particles emerging from the other. Careful manipulation of the inlet and outlet flow rates and the field strength enables the cut-off diameter of the particles to be selected.