hybridization assay

https://doi.org/10.1351/goldbook.09870

Assay with specifically designed single-stranded deoxyribonucleic acid (DNA) probe with a defined (known) nucleotide sequence usually immobilised on a surface (in such a case, the nucleic acid probe is called the capture probe).

Note: The probe is used as a recognition element to test for the nucleotide sequence within the target DNA in the sample solution. If target DNA contains a sequence complementary to the probe, a hybrid double-stranded DNA (dsDNA) is formed.
Source:
PAC, 2018, 90, 1121. (Terminology of bioanalytical methods (IUPAC Recommendations 2018)) on page 1184 [Terms] [Paper]
Citation: 'hybridization assay' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. https://doi.org/10.1351/goldbook.09870 RIS BibTex EndNote
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