two-dimensional gel electrophoresis

in proteomics
initialism: 2D PAGE
https://doi.org/10.1351/goldbook.09788

Technique used to separate proteins using isoelectric focusing in a gel. The proteins are then further separated in the second dimension using denaturing PAGE.

Note: 2D PAGE greatly enhances the capacity of the separation and facilitates further analysis, such as the difference gel electrophoresis or the mass spectrometric analysis of gel separated proteins.
Source:
PAC, 2018, 90, 1121. (Terminology of bioanalytical methods (IUPAC Recommendations 2018)) on page 1165 [Terms] [Paper]
Citation: 'two-dimensional gel electrophoresis' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. https://doi.org/10.1351/goldbook.09788 RIS BibTex EndNote
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