Protein cleavage to generate proteolytic peptides for bottom-up proteomic analysis, either enzymatic or chemical.
Note: Both enzymatic and chemical proteolysis may be sequence-specific or unspecific. Proteolysis is specific where cleavage appears at a few specific sequence motives (e.g. proteolysis by trypsin), or unspecific, being connected to wide range of sequence motives (e.g. proteolysis by chymotrypsin).
Source:
PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1162 (https://doi.org/10.1515/pac-2016-1120)