The separation of two peaks in terms of their average peak width at base (t R2 > t R1): \[R_{\text{s}}=\frac{t_{\text{R2}}\,-\,t_{\text{R1}}}{\frac{w_{\text{b1}}\,+\,w_{\text{b2}}}{2}}=\frac{2\ (t_{\text{R2}}- t_{\text{R1}})}{w_{\text{b1}}\,+\,w_{\text{b2}}}\] In the case of two adjacent peaks it may be assumed that w b1 ≈ w b2, and thus, the width of the second peak may be substituted for the average value: \[R_{\text{s}}\approx \frac{t_{\text{R2}}\,-\,t_{\text{R1}}}{w_{\text{b2}}}\]