Title: Lineweaver–Burk plot
Long Title: IUPAC Gold Book - Lineweaver–Burk plot
DOI: 10.1351/goldbook.L03566
Status: current

Definition
A plot of the reciprocal of velocity of an enzyme-catalysed reaction (ordinate) versus the reciprocal of substrate concentration (abscissa). The plot is used to graphically define the maximum velocity of an enzyme-catalysed reaction and the Michaelis constant for the enzyme.

Related Terms
- Michaelis constant: https://goldbook.iupac.org//terms/view/M03891
- Michaelis–Menten kinetics: https://goldbook.iupac.org//terms/view/M03892

Source
- PAC, 1994, 66, 2587. 'Glossary of bioanalytical nomenclature - Part 1: General terminology, body fluids, enzymology, immunology (IUPAC Recommendations 1994)' on page 2593 (https://doi.org/10.1351/pac199466122587)

Other Outputs
- html: https://goldbook.iupac.org/terms/view/L03566/html
- json: https://goldbook.iupac.org/terms/view/L03566/json
- xml: https://goldbook.iupac.org/terms/view/L03566/xml

Citation: Citation: 'Lineweaver–Burk plot' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.L03566
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Accessed: 2025-12-24T00:15:29+00:00