Title: column chromatography Long Title: IUPAC Gold Book - column chromatography DOI: 10.1351/goldbook.C01182 Status: current Definition A separation technique in which the stationary bed is within a tube. The particles of the solid stationary phase or support coated with a liquid stationary phase may fill the whole inside volume of the tube (packed column) or be concentrated on or along the inside tube wall leaving an open, unrestricted path for the mobile phase in the middle part of the tube (open-tubular column). Source - PAC, 1993, 65, 819. 'Nomenclature for chromatography (IUPAC Recommendations 1993)' on page 825 (https://doi.org/10.1351/pac199365040819) Other Outputs - html: https://goldbook.iupac.org/terms/view/C01182/html - json: https://goldbook.iupac.org/terms/view/C01182/json - xml: https://goldbook.iupac.org/terms/view/C01182/xml Citation: Citation: 'column chromatography' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.C01182 License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/) for individual terms. Collection: If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org . Disclaimer: The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using. Accessed: 2026-05-10T19:29:04+00:00