https://doi.org/10.1351/goldbook.14198
Spectroscopic technique that discriminates between labeled molecules in the homogeneous phase and those interacting with binding partners immobilized at a surface.
Note: TIRF measurements are restricted to a thin layer and are achieved by exciting the fluorophore with an evanescent wave, created by total internal reflection at the glass/aqueous interface of the wave guide. The goal of using TIRF in biological applications is to study events close to the interface of two different media.