14190 IUPAC Gold Book - screen validation 10.1351/goldbook.14190 14190 current 1 Assay conditions, as determined by assay validation, are performed in the chosen plate format with an acceptable signal to background ratio as described by the Z′ factor. Validation typically requires testing a number of samples a minimum of three times and allows for the estimation of false positives, false negatives, and the selection of statistically significant thresholds. assay validation https://goldbook.iupac.org//terms/view/14109 plate format https://goldbook.iupac.org//terms/view/14176 PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1154 (https://doi.org/10.1351/PAC-REC-09-05-03) https://goldbook.iupac.org/terms/view/14190/html https://goldbook.iupac.org/terms/view/14190/json https://goldbook.iupac.org/terms/view/14190/plain Citation: 'screen validation' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.14190 The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/) for individual terms. If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org . The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using. 2026-07-24T15:55:53+00:00