14168 IUPAC Gold Book - negative assay control 10.1351/goldbook.14168 14168 current synonym: low control 1 Experimental conditions designed to produce the signal reflective of the absence of the process under investigation. For a binding or an activation mechanism, it is typically the signal measured in the absence of a test compound and is often relevant to the determination of the background signal. For an inhibitory or antagonist mechanism, it is the maximal signal, obtained in the absence of the reference inhibitor. PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1149 (https://doi.org/10.1351/PAC-REC-09-05-03) https://goldbook.iupac.org/terms/view/14168/html https://goldbook.iupac.org/terms/view/14168/json https://goldbook.iupac.org/terms/view/14168/plain Citation: 'negative assay control' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.14168 The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/) for individual terms. If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org . The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using. 2026-06-04T08:39:44+00:00