14148 IUPAC Gold Book - image-based assay 10.1351/goldbook.14148 14148 current 1 Assay in which image analysis algorithms that determine the compartmentalization of fluorescent probes give topological as well as quantitative information. The use of automated cellular imaging for screening has been made possible by the development of digital imaging technology. high-content screening https://goldbook.iupac.org//terms/view/14141 PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1143 (https://doi.org/10.1351/PAC-REC-09-05-03) https://goldbook.iupac.org/terms/view/14148/html https://goldbook.iupac.org/terms/view/14148/json https://goldbook.iupac.org/terms/view/14148/plain Citation: 'image-based assay' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.14148 The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/) for individual terms. If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org . The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using. 2026-04-17T21:11:23+00:00