https://doi.org/10.1351/goldbook.14137
Assay based on the nonradiative energy transfer that occurs when the emission spectrum of a fluorescent donor molecule overlaps with the absorption spectrum of an energy acceptor molecule.
Notes:
- The donor and the acceptor fluorophores are linked directly or indirectly to two binding partners, and energy transfer occurs when the fluorophores are in close proximity with proper alignment of donor and acceptor electronic dipoles, i.e., when the binding partners interact.
- The fluorophores can be either of small molecule or protein nature.
- Use of a donor with a long fluorescence lifetime allows time-delayed (or time-resolved) fluorescence measurement (TR-FRET), providing conditions where other fluorophores in the assay medium are not detected.