Title: assay validation
Long Title: IUPAC Gold Book - assay validation
DOI: 10.1351/goldbook.14109
Status: current

Definition
Experiments conducted to verify that the output measurements of the assay are consistently reflective of the activity against the target.

Note
Results are compared internally over multiple runs and externally (when available) to existing literature parameters such as \(K_{\rm{d}}\), \(K_{\rm{i}}\), \(K_{\rm{m}}\), or \(\pu{EC_{50}}\).

Source
- PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1132 (https://doi.org/10.1351/PAC-REC-09-05-03)

Other Outputs
- html: https://goldbook.iupac.org/terms/view/14109/html
- json: https://goldbook.iupac.org/terms/view/14109/json
- xml: https://goldbook.iupac.org/terms/view/14109/xml

Citation: Citation: 'assay validation' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.14109
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Disclaimer: The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using.
Accessed: 2026-06-28T09:06:59+00:00