{"term":{"id":"14109","title":"assay validation","longtitle":"IUPAC Gold Book - assay validation","doi":"10.1351\/goldbook.14109","code":"14109","status":"current","definitions":[{"id":1,"text":"Experiments conducted to verify that the output measurements of the assay are consistently reflective of the activity against the target.","notes":{"1":"Results are compared internally over multiple runs and externally (when available) to existing literature parameters such as \\(K_{\\rm{d}}\\), \\(K_{\\rm{i}}\\), \\(K_{\\rm{m}}\\), or \\(\\pu{EC_{50}}\\)."},"sources":["PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1132 (https:\/\/doi.org\/10.1351\/PAC-REC-09-05-03)"]}],"altoutputs":{"html":"https:\/\/goldbook.iupac.org\/terms\/view\/14109\/html","xml":"https:\/\/goldbook.iupac.org\/terms\/view\/14109\/xml","plain":"https:\/\/goldbook.iupac.org\/terms\/view\/14109\/plain"},"citation":"Citation: 'assay validation' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351\/goldbook.14109","license":"The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https:\/\/creativecommons.org\/licenses\/by-sa\/4.0\/) for individual terms.","collection":"If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org .","disclaimer":"The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using.","accessed":"2026-06-28T09:06:43+00:00"}}