https://doi.org/10.1351/goldbook.13853
Equation used to calculate the association constant, \(K_{\rm{a}}\), of a ligand with a protein molecule.
Notes:
- The Scatchard equation is \[\frac{r}{c} = -K_{\rm{a}} r + K_{\rm{a}} n\] in which \(r\) is the ratio of the concentration of bound ligand to the concentration of available protein binding sites, \(c\) is the concentration of free ligand, and \(n\) is the number of binding sites per protein molecule.
- The association constant, \(K_{\rm{a}}\), has units of reciprocal concentration.