Title: delayed-type hypersensitivity assay
Long Title: IUPAC Gold Book - delayed-type hypersensitivity assay
DOI: 10.1351/goldbook.13086
Status: current

Definition
In vivo assay of cell-mediated immune response, elicited by antigen in the skin and mediated by CD4+ Th1 cells.

Note
DTH reactions are often divided into two phases which should be assessed in the assay: the sensitization phase, referring to the initial immunization with specific antigen, and the efferent or challenge phase of the DTH response, which usually follows 6 to 14 days after sensitization.

Related Terms
- CD4: https://goldbook.iupac.org//terms/view/12973
- Th1 cells: https://goldbook.iupac.org//terms/view/13718
- cell-mediated immune response: https://goldbook.iupac.org//terms/view/12994
- challenge: https://goldbook.iupac.org//terms/view/12997

Source
- PAC, 2012, 84, 1113. 'IUPAC glossary of terms used in immunotoxicology (IUPAC Recommendations 2012)' on page 1156 (https://doi.org/10.1351/PAC-REC-11-06-03)

Other Outputs
- html: https://goldbook.iupac.org/terms/view/13086/html
- json: https://goldbook.iupac.org/terms/view/13086/json
- xml: https://goldbook.iupac.org/terms/view/13086/xml

Citation: Citation: 'delayed-type hypersensitivity assay' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.13086
License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/) for individual terms.
Collection: If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org .
Disclaimer: The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using.
Accessed: 2026-07-27T21:07:40+00:00