antibody-forming cell assay

initialism: AFC
synonyms: hemolytic plaque assay, Jerne plaque assay, plaque-forming cell (PFC) assay, AFC assay
https://doi.org/10.1351/goldbook.12880
Assay that measures the humoral immune response mediated by the concerted actions of antigen-presenting cells, T lymphocytes, and B lymphocytes, generally by determination of murine primary IgM or IgG antibodies directed against the T-cell-dependent sheep red blood cell (SRBC) surface antigens after in vivo sensitization.
Note: Due to involvement of multiple cell populations in mounting an antibody response, the AFC assay actually evaluates several immune parameters simultaneously. It is considered to be one of the most sensitive indicator systems for immunotoxicology studies.
Source:
PAC, 2012, 84, 1113. (IUPAC glossary of terms used in immunotoxicology (IUPAC Recommendations 2012)) on page 1124 [Terms] [Paper]
Citation: 'antibody-forming cell assay' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. https://doi.org/10.1351/goldbook.12880 RIS BibTex EndNote
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