10039 IUPAC Gold Book - total retention time 10.1351/goldbook.10039 10039 current 1 Time between sample injection and the emergence of the peak maximum of the sample component of interest. Total retention time includes the hold-up time. The total retention time is related to the total retention volume (\(V_{\rm{R}}\)) and flow rate (\(F_{\rm{c}}\)) by \(t_{\rm{R}} = V_{\rm{R}}⁄F_{\rm{c}}\) . hold-up time https://goldbook.iupac.org//terms/view/10038 PAC, 2017, 90, 181. 'Terminology of separation methods (IUPAC Recommendations 2017)' on page 199 (https://doi.org/10.1515/pac-2017-0111) https://goldbook.iupac.org/terms/view/10039/html https://goldbook.iupac.org/terms/view/10039/json https://goldbook.iupac.org/terms/view/10039/plain Citation: 'total retention time' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.10039 The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/) for individual terms. If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org . The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using. 2026-05-18T05:28:20+00:00