Title: hold-up time Long Title: IUPAC Gold Book - hold-up time DOI: 10.1351/goldbook.10038 Status: current Definition Time required to elute a component which is not retained by the stationary phase. Notes 1) The hold-up time includes any time that the component spends in the sample injector, the detector, and connectors. 2) The hold-up time is related to the hold-up volume (\(V_{\rm{M}}\)) and flow rate (\(F_{\rm{c}}\)) by \(t_{\rm{M}} = V_{\rm{M}}⁄F_{\rm{c}}\) . Source - PAC, 2017, 90, 181. 'Terminology of separation methods (IUPAC Recommendations 2017)' on page 196 (https://doi.org/10.1515/pac-2017-0111) Other Outputs - html: https://goldbook.iupac.org/terms/view/10038/html - json: https://goldbook.iupac.org/terms/view/10038/json - xml: https://goldbook.iupac.org/terms/view/10038/xml Citation: Citation: 'hold-up time' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.10038 License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/) for individual terms. Collection: If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org . Disclaimer: The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using. Accessed: 2026-05-18T06:29:02+00:00