Field-flow fractionation in which the physical force field is a centrifugal force field.
Notes: - SdFFF is performed in a circular flat channel inserted inside a centrifugal basket.
- SdFFF can be applied to a wide range of colloidal and micron size particles and is a powerful method for cell separations, including industrial carbon blacks, silica nanoparticles, silver and gold nano-particles, aquatic sediments, aerosols, and latex beads.
- SdFFF can be successfully applied to the fractionation and characterization (sizing) of submicron particles, starting from about \(\pu{5 nm}\), but this limit depends on the particle density, as the separation is based on the buoyant mass of the particles.
- SdFFF can operate in both the Brownian field-flow fractionation (BFFF) and hyperlayer field-flow fractionation (HyFFF) modes and caution must be exercised if samples span the Brownian-hyperlayer transition point of about \(\pu{1 \upmu\!m}\), as this can lead to separated fractions containing two particle sizes, smaller particles separated by Brownian mode and larger particles separated by hyperlayer mode.
Source:
PAC, 2017, 90, 181. 'Terminology of separation methods (IUPAC Recommendations 2017)' on page 222 (https://doi.org/10.1515/pac-2017-0111)