Title: hydrophobic-interaction chromatography
Long Title: IUPAC Gold Book - hydrophobic-interaction chromatography
DOI: 10.1351/goldbook.09891
Status: current

Definition
Chromatography in which molecules are separated by interactions between their hydrophobic moieties and hydrophobic sites on the surface of the stationary phase.

Note
High salt concentrations are used in the mobile phase and separations are effected by changing the salt concentration. Gradients are run by decreasing the salt concentration over time.

Source
- PAC, 2017, 90, 181. 'Terminology of separation methods (IUPAC Recommendations 2017)' on page 187 (https://doi.org/10.1515/pac-2017-0111)

Other Outputs
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- xml: https://goldbook.iupac.org/terms/view/09891/xml

Citation: Citation: 'hydrophobic-interaction chromatography' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.09891
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Accessed: 2026-05-18T08:16:04+00:00