Treatment with alkaline solution (e.g. hydrazine,
\(\ce{KOH}\),
\(\ce{NaOH}\)) useful for the cleavage of fatty acids.
Note: The resulting (partially or fully) O-deacylated and (fully) N-deacylated products enable the determination of the fatty acid substitution positions by mass spectrometry.
Source:
PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1176 (https://doi.org/10.1515/pac-2016-1120)