Analytical methods and related techniques to measure the protein content.
Notes: - Protein quantification can be further divided into relative quantification and absolute quantification. Basic analytical methods used in proteomics to quantify protein content are immunoanalysis (see Western blotting), mass spectrometry or densitometry of differential staining. Especially mass spectrometry offers many techniques based either on stable isotope labelling or label-free approaches.
- Absolute quantification of proteins (AQUA) is performed via measurements on constituent proteolytic peptides using chemically synthesised isotope-labelled peptides as surrogate internal standards for mass spectrometry.
Source:
PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1161 (https://doi.org/10.1515/pac-2016-1120)