Title: protein complex purification
Long Title: IUPAC Gold Book - protein complex purification
DOI: 10.1351/goldbook.09763
Status: current

Definition
Set of methods serving to purify the intact protein complex for further analysis.

Note
The methods used may employ single step affinity purification or tandem affinity purification (two affinity purification steps). These approaches include the direct application of an epitope on a bait protein for immunopurification or the genetic insertion of an affinity tag to bait protein, both used to co-purify the prey components of the protein complex.

Related Term
- protein complex: https://goldbook.iupac.org//terms/view/09762

Source
- PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1160 (https://doi.org/10.1515/pac-2016-1120)

Other Outputs
- html: https://goldbook.iupac.org/terms/view/09763/html
- json: https://goldbook.iupac.org/terms/view/09763/json
- xml: https://goldbook.iupac.org/terms/view/09763/xml

Citation: Citation: 'protein complex purification' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.09763
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Accessed: 2026-05-28T13:02:29+00:00