{"term":{"id":"09763","title":"protein complex purification","longtitle":"IUPAC Gold Book - protein complex purification","doi":"10.1351\/goldbook.09763","code":"09763","status":"current","definitions":[{"id":1,"text":"Set of methods serving to purify the intact protein complex for further analysis.","notes":{"1":"The methods used may employ single step affinity purification or tandem affinity purification (two affinity purification steps). These approaches include the direct application of an epitope on a bait protein for immunopurification or the genetic insertion of an affinity tag to bait protein, both used to co-purify the prey components of the protein complex."},"links":[{"term":"protein complex","url":"https:\/\/goldbook.iupac.org\/\/terms\/view\/09762"}],"sources":["PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1160 (https:\/\/doi.org\/10.1515\/pac-2016-1120)"]}],"altoutputs":{"html":"https:\/\/goldbook.iupac.org\/terms\/view\/09763\/html","xml":"https:\/\/goldbook.iupac.org\/terms\/view\/09763\/xml","plain":"https:\/\/goldbook.iupac.org\/terms\/view\/09763\/plain"},"citation":"Citation: 'protein complex purification' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351\/goldbook.09763","license":"The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https:\/\/creativecommons.org\/licenses\/by-sa\/4.0\/) for individual terms.","collection":"If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org .","disclaimer":"The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using.","accessed":"2026-05-28T13:01:42+00:00"}}