09752 IUPAC Gold Book - multidimensional protein identification technology 10.1351/goldbook.09752 09752 current initialism: MudPIT 1 Non-gel step-wise multidimensional technique for separating and identifying individual components of complex protein and peptide mixtures. It combines fractionation of proteolytic peptides in steps on a strong cation exchanger (SAX) using ion exchange chromatography (IEC); each step uses higher elution force. Then, each fraction is separated on reversed phase liquid chromatography and analysed using mass spectrometry. PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1157 (https://doi.org/10.1515/pac-2016-1120) https://goldbook.iupac.org/terms/view/09752/html https://goldbook.iupac.org/terms/view/09752/json https://goldbook.iupac.org/terms/view/09752/plain Citation: 'multidimensional protein identification technology' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.09752 The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/) for individual terms. If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org . The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using. 2026-08-02T11:07:07+00:00