Title: multidimensional protein identification technology
Long Title: IUPAC Gold Book - multidimensional protein identification technology
DOI: 10.1351/goldbook.09752
Status: current

Definition
Non-gel step-wise multidimensional technique for separating and identifying individual components of complex protein and peptide mixtures. It combines fractionation of proteolytic peptides in steps on a strong cation exchanger (SAX) using ion exchange chromatography (IEC); each step uses higher elution force. Then, each fraction is separated on reversed phase liquid chromatography and analysed using mass spectrometry.

Source
- PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1157 (https://doi.org/10.1515/pac-2016-1120)

Other Outputs
- html: https://goldbook.iupac.org/terms/view/09752/html
- json: https://goldbook.iupac.org/terms/view/09752/json
- xml: https://goldbook.iupac.org/terms/view/09752/xml

Citation: Citation: 'multidimensional protein identification technology' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.09752
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Accessed: 2026-08-03T13:15:19+00:00