Title: Sanger sequencing
Long Title: IUPAC Gold Book - Sanger sequencing
DOI: 10.1351/goldbook.09725
Status: current

Definition
Method for determining a nucleotide sequence of deoxyribonucleic acid (DNA) based on incorporating chain-terminating dideoxynucleotides.

Note
The method is named after Frederick Sanger (1918–2013, awarded the Nobel Prize in 1958 and 1980).

Related Term
- nucleotide sequence: https://goldbook.iupac.org//terms/view/09712

Source
- PAC, 2018, 90, 1121. 'Terminology of bioanalytical methods (IUPAC Recommendations 2018)' on page 1152 (https://doi.org/10.1515/pac-2016-1120)

Other Outputs
- html: https://goldbook.iupac.org/terms/view/09725/html
- json: https://goldbook.iupac.org/terms/view/09725/json
- xml: https://goldbook.iupac.org/terms/view/09725/xml

Citation: Citation: 'Sanger sequencing' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.09725
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Disclaimer: The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using.
Accessed: 2025-09-24T10:12:31+00:00