immunoblotting

https://doi.org/10.1351/goldbook.09671
Technique that uses antibodies (or other specific ligands in related techniques) to identify target proteins among a number of unrelated protein species.
Note: The techniques allow the detection of proteins from complex mixtures separated by gel chromatography and then transferred to a membrane. After incubation of the attached product from membrane with labelled antibody, the target proteins become visible.
Source:
PAC, 2018, 90, 1121. (Terminology of bioanalytical methods (IUPAC Recommendations 2018)) on page 1139 [Terms] [Paper]
Citation: 'immunoblotting' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. https://doi.org/10.1351/goldbook.09671 RIS BibTex EndNote
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