Title: 15N-NOESY-HSQC
Long Title: IUPAC Gold Book - <sup>15</sup>N-NOESY-HSQC
DOI: 10.1351/goldbook.08308
Status: current

Definition
Three-dimensional nuclear magnetic resonance spectroscopy for the assignment of protein structure with one nitrogen and two hydrogen dimensions.

Note
Magnetization is exchanged between all hydrogens using the nuclear Overhauser effect before being transferred to neighbouring \(\ce{^{15}N}\) nuclei and then back to \(\ce{^{1}H}\) for detection. This spectrum can be used to obtain restraints for structure calculations.

Related Terms
- Three-dimensional nuclear magnetic resonance spectroscopy: https://goldbook.iupac.org//terms/view/08432
- nuclear Overhauser effect: https://goldbook.iupac.org//terms/view/08385
- spectrum: https://goldbook.iupac.org//terms/view/08384

Source
- PAC, 2021, 93, 647. 'Glossary of methods and terms used in analytical spectroscopy (IUPAC Recommendations 2019)' on page 680 (https://doi.org/10.1515/pac-2019-0203)

Other Outputs
- html: https://goldbook.iupac.org/terms/view/08308/html
- json: https://goldbook.iupac.org/terms/view/08308/json
- xml: https://goldbook.iupac.org/terms/view/08308/xml

Citation: Citation: '<sup>15</sup>N-NOESY-HSQC' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.08308
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Accessed: 2026-05-30T20:15:53+00:00