Title: 13C-HMQC-NOESY
Long Title: IUPAC Gold Book - <sup>13</sup>C-HMQC-NOESY
DOI: 10.1351/goldbook.08306
Status: current

Definition
Three-dimensional nuclear magnetic resonance spectroscopy for the assignment of protein structure with one carbon and two hydrogen dimensions.

Notes
1) In the initial HMQC step, magnetization is transferred from \(\ce{^{1}H}\) to \(\ce{^{13}C}\) and back again. This is then followed by a NOESY step in which the magnetization is transferred to any other hydrogen nucleus close by before detection.
2) The technique requires \(\ce{^{13}C}\) labelling.
3) The spectrum is used to obtain restraints for structure calculations.

Related Terms
- NOESY: https://goldbook.iupac.org//terms/view/08387
- Three-dimensional nuclear magnetic resonance spectroscopy: https://goldbook.iupac.org//terms/view/08432
- spectrum: https://goldbook.iupac.org//terms/view/08384

Source
- PAC, 2021, 93, 647. 'Glossary of methods and terms used in analytical spectroscopy (IUPAC Recommendations 2019)' on page 680 (https://doi.org/10.1515/pac-2019-0203)

Other Outputs
- html: https://goldbook.iupac.org/terms/view/08306/html
- json: https://goldbook.iupac.org/terms/view/08306/json
- xml: https://goldbook.iupac.org/terms/view/08306/xml

Citation: Citation: '<sup>13</sup>C-HMQC-NOESY' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.08306
License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/) for individual terms.
Collection: If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org .
Disclaimer: The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using.
Accessed: 2026-05-30T21:53:56+00:00